Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
Mission Bio
39:56
Genetic variants,both coding and noncoding,can profoundly influence gene regulation and disease progression. Yet, systematically linking genetic variants to functional consequences with single-cell resolution remains a major challenge.
In this webinar, Dr. Dominik Lindenhofer (EMBL Heidelberg) will present his pioneering work on single-cell DNA–RNA sequencing (SDR-seq), a breakthrough method developed to simultaneously detect hundreds of genomic variants and transcripts within the same cell. By applying SDR-seq to induced pluripotent stem cells and B cell lymphoma samples, Dr. Lindenhofer demonstrates how coding and noncoding genetic variants influence gene expression and tumorigenic signaling. SDR-seq has major clinical applications because it can help pinpoint how specific genetic variants alter gene expression to guide more precise diagnostics and targeted therapeutic strategies through single-cell analysis.
Speaker
Dr Dominik Lindenhofer
Research Scientist
Dr. Dominik Lindenhofer is a postdoctoral researcher at the European Molecular Biology Laboratory in Heidelberg, where he works in Lars Steinmetz’s group in the Genome Biology Unit. His research focuses on developing and applying cutting-edge single-cell and multi-omics technologies to understand how genetic variants shape gene regulation in development and disease.
During his PhD at the Institute of Molecular Biotechnology in Vienna with Jürgen Knoblich, Dr. Lindenhofer pioneered CRISPR-based approaches in human cerebral organoids, leading to high-impact discoveries on brain development and size regulation, including publications in Science and Nature Cell Biology.
At EMBL, he has expanded his focus to the cardiovascular system, advancing methods such as SDR-seq to directly assess the functional impact of non-coding variants, and applying high-throughput single-cell perturbation screens in heart organoids. His work has been recognized through a prestigious HFSP Long-Term Fellowship and invited talks at leading international meetings.
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Functional phenotyping of genomic variants using joint multiomic single-cell DNA–RNA sequencing
39:56